A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles
read more Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters.Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.In this protocol, we describe the incorporation of phospholipid scramblases into giant unilamellar vesicles (GUV